E. coli protein production system

E. coli production system  

Bioneer has many years of experience with E. coli fermentations and can offer a customised strategy tailored to suit your requirements.  

The services we offer include:

• Codon optimisation of your gene of interest
• Cloning of the native and/or optimised gene in an appropriate vector
• Benchmarking several E. coli expression systems with respect to protein yield and quality
• Testing of critical features, e.g. selection markers and affinity tags as well as intracellular and periplasmic expression
• Initial estimation of yield by standard protein analysis
• Creation of a Research Master Cell Bank

For further information, please contact Group Leader, Bacterial Expression; Søren Madsen by phone (+45 45160444) or email.