Caco-2 cells
Drug transport across the Caco-2 cell monolayer
Bioneer:FARMA assess the intestinal permeability of your compound using Caco-2 cell culture.
The Caco-2 cell culture model is widely used for prediction of the transport of a compound across the intestinal wall. The apparent permeability of compound can be determined, and by comparison with the permeability of marker compounds, the permeability of a specific compound can be classified as high or low.
The Caco-2 model also offers the ability to describe whether drug transporters may be involved in the absorption process, either aiding or inhibiting drug absorption. By performing unidirectional transport assessments Bioneer:FARMA determines the flux of drug from the apical to basolateral compartment and thereby the permeability.
Utilising bi-directional transport assessments Bioneer:FARMA elucidates the possible involvement of transporter or efflux transporters in the absorption of a given compound. Furthermore, we specifically determine whether your compound undergoes P-glycoprotein (P-gp) mediated active efflux, by including substrate or inhibitors of P-gp in the assays. In general the offered studies can be performed either using standard buffer vehicles or using biorelevant media.
The Caco-2 cell culture is a well recognised model of the small intestinal epithelial cell barrier. Caco-2 cells are derived from human colonic carcinoma cells and have the ability to form monolayers when grown on Transwell filters. The monolayer is polarized, having a brush border membrane on the apical side and tight junctions between the cells, thus resembling the physiology of the intestinal epithelium. The Caco-2 cells are grown on Transwell filters for 21 days in order to secure proper monolayer formation and cell differentiation.
Bioneer:FARMA assess the intestinal permeability of your compound using Caco-2 cell culture.
The Caco-2 cell culture model is widely used for prediction of the transport of a compound across the intestinal wall. The apparent permeability of compound can be determined, and by comparison with the permeability of marker compounds, the permeability of a specific compound can be classified as high or low.
The Caco-2 model also offers the ability to describe whether drug transporters may be involved in the absorption process, either aiding or inhibiting drug absorption. By performing unidirectional transport assessments Bioneer:FARMA determines the flux of drug from the apical to basolateral compartment and thereby the permeability.
Utilising bi-directional transport assessments Bioneer:FARMA elucidates the possible involvement of transporter or efflux transporters in the absorption of a given compound. Furthermore, we specifically determine whether your compound undergoes P-glycoprotein (P-gp) mediated active efflux, by including substrate or inhibitors of P-gp in the assays. In general the offered studies can be performed either using standard buffer vehicles or using biorelevant media.
The Caco-2 cell culture is a well recognised model of the small intestinal epithelial cell barrier. Caco-2 cells are derived from human colonic carcinoma cells and have the ability to form monolayers when grown on Transwell filters. The monolayer is polarized, having a brush border membrane on the apical side and tight junctions between the cells, thus resembling the physiology of the intestinal epithelium. The Caco-2 cells are grown on Transwell filters for 21 days in order to secure proper monolayer formation and cell differentiation.